NELFE Polyclonal antibody

• Featured Product
• KD/KO Validated

NELFE Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 10705-1-AP

Synonyms

RNA-binding protein RD, RDBP, RD, NELF-E, NELF E

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Validation Data Gallery

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  WB analysis of HEK-293 using 10705-1-AP

  WB result of NELFE antibody (10705-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFE transfected HEK-293 cells.

• 

  WB analysis using 10705-1-AP

  Various lyastes were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10705-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 10705-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 10705-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10705-1-AP

  IP result of anti-NELFE (IP:10705-1-AP, 4ug; Detection:10705-1-AP 1:6000) with mouse brain tissue lysate 1840 ug.

• 

  IHC staining of human prostate cancer using 10705-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10705-1-AP (NELFE antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 10705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NELFE antibody (10705-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of hela using 10705-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NELFE (10705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, mouse kidney tissue, rat kidney tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ChIP: See 4 publications below
• RIP: See 1 publications below

Product Information

10705-1-AP targets NELFE in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NELFE fusion protein Ag1186
• Full Name: RD RNA binding protein
• Calculated Molecular Weight: 37 kDa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC011600
• Gene Symbol: NELFE
• Gene ID (NCBI): 7936
• RRID: AB_513966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

Publications

Species	Application	Title
mouse	IF	Nature; The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.; Authors - Bofeng Liu
human, mouse	WB	Cell; Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.; Authors - Sawarkar Ritwick R
mouse	WB,chIP	Nat Commun; Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.; Authors - Li Yu
mouse	WB	Nat Commun; RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.; Authors - Bogang Wu
mouse	WB	Dev Cell; Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.; Authors - Daniel C L Robinson
mouse	WB,IHC	Proc Natl Acad Sci U S A; Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.; Authors - Adelman Karen K

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

HUAI-CHIN (Verified Customer) (02-14-2019)

This antibody works great in Western. Gives me clean single band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse embryonic fibroblast

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