RCN2 Recombinant antibody

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  WB analysis using 84956-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84956-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84956-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 84956-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RCN2 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84956-4-RR

Clone No.242612A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

E6BP, E6-binding protein, E6 binding protein, Calcium-binding protein ERC-55, Calcium binding protein ERC 55

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Tested Applications

• Positive WB detected in: rat liver tissue, HeLa cells, HEK-293 cells, U-251 cells, mouse liver tissue
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84956-4-RR targets RCN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RCN2 fusion protein Ag0247
• Full Name: reticulocalbin 2, EF-hand calcium binding domain
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC004892
• Gene Symbol: RCN2
• Gene ID (NCBI): 5955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14257
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.