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CoraLite®594-conjugated RCC1 Monoclonal antibody

RCC1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C2F5

Cat no : CL594-67335

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated RCC1 Monoclonal antibody (CL594-67335)

    IF Staining of HepG2 using CL594-67335

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RCC1 antibody (CL594-67335, Clone: 2C2F5 ) at dilution of 1:200. DAPI (blue).

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67335 targets RCC1 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RCC1 fusion protein Ag17809
Full Name regulator of chromosome condensation 1
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_2920115
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

Protocols

Product Specific Protocols
IF protocol for CL594 RCC1 antibody CL594-67335Download protocol
Standard Protocols
Click here to view our Standard Protocols