CoraLite®594-conjugated RCAS1 Monoclonal antibody

RCAS1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4H8A12

Cat no : CL594-66170

Synonyms

EB9, EBAG9, PDAF, RCAS1

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66170

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 RCAS1 antibody (CL594-66170, Clone: 4H8A12 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66170 targets RCAS1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RCAS1 fusion protein Ag2905
• Full Name: estrogen receptor binding site associated, antigen, 9
• Calculated Molecular Weight: 213 aa, 24 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC017729
• Gene Symbol: RCAS1
• Gene ID (NCBI): 9166
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.