RBM17 Recombinant antibody, PBS Only

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  WB analysis using 82889-3-RR (same clone as 82889-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82889-3-RR (RBM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

  WB result of RBM17 antibody (82889-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

  IP result of anti-RBM17 (IP:82889-3-RR, 4ug; Detection:82889-3-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82889-3-RR (same clone as 82889-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82889-3-RR (RBM17 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82889-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82889-3-RR against Human RBM17 were performed. The affinity constant is 0.828 nM.

• Featured Product
• KD/KO Validated

RBM17 {ptg:Brand} Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 82889-3-PBS

Clone No.230125A3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

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Product Information

82889-3-PBS targets RBM17 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RBM17 fusion protein Ag0985
• Full Name: RNA binding motif protein 17
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC007871
• Gene Symbol: RBM17
• Gene ID (NCBI): 84991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96I25
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.