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  • Featured Product
  • KD/KO Validated

RBM17 Recombinant antibody

RBM17 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

230125A3

Cat no : 82889-3-RR

Synonyms

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM17 Recombinant antibody (82889-3-RR)

    WB analysis using 82889-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82889-3-RR (RBM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RBM17 Recombinant antibody (82889-3-RR)

    WB analysis of MCF-7 using 82889-3-RR

    WB result of RBM17 antibody (82889-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RBM17 Recombinant antibody (82889-3-RR)

    IP experiment of MCF-7 using 82889-3-RR

    IP result of anti-RBM17 (IP:82889-3-RR, 4ug; Detection:82889-3-RR 1:10000) with MCF-7 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using RBM17 Recombinant antibody (82889-3-RR)

    IHC staining of human ovarian cancer using 82889-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82889-3-RR (RBM17 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82889-3-RR

    Affinity and Kinetic Characterization of 82889-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-3-RR against Human RBM17 were performed. The affinity constant is 0.828 nM.

Tested Applications

Positive WB detected inBxPC-3 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82889-3-RR targets RBM17 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBM17 fusion protein Ag0985
Full Name RNA binding motif protein 17
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC007871
Gene Symbol RBM17
Gene ID (NCBI) 84991
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.

Protocols

Product Specific Protocols
WB protocol for RBM17 antibody 82889-3-RRDownload protocol
IHC protocol for RBM17 antibody 82889-3-RRDownload protocol
IP protocol for RBM17 antibody 82889-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols