RBM15 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RBM15 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4A1A4

Cat no : 66059-1-PBS

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Validation Data Gallery

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  WB analysis using 66059-1-Ig (same clone as 66059-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK293 cells using 66059-1-Ig (same clone as 66059-1-PBS)

  WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66059-1-Ig (same clone as 66059-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66059-1-Ig (same clone as 66059-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66059-1-Ig (same clone as 66059-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 66059-1-Ig (same clone as 66059-1-PBS)

  IP result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66059-1-Ig (same clone as 66059-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66059-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66059-1-PBS targets RBM15 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RBM15 fusion protein Ag17970
• Full Name: RNA binding motif protein 15
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC006397
• Gene Symbol: RBM15
• Gene ID (NCBI): 64783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.