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  • Featured Product
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RBM15 Monoclonal antibody

RBM15 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4A1A4

Cat no : 66059-1-Ig

Synonyms

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis using 66059-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis of HeLa using 66059-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK293 cells using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis of HEK293 cells using 66059-1-Ig

    WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells.

  • Western Blot (WB) analysis of human brain tissue using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis of human brain using 66059-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis of HeLa using 66059-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis of human heart using 66059-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RBM15 Monoclonal antibody (66059-1-Ig)

    WB analysis of HEK-293 using 66059-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RBM15 Monoclonal antibody (66059-1-Ig)

    IP experiment of HEK-293 using 66059-1-Ig

    IP result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RBM15 Monoclonal antibody (66059-1-Ig)

    IHC staining of human breast cancer using 66059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBM15 Monoclonal antibody (66059-1-Ig)

    IHC staining of human liver cancer using 66059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBM15 Monoclonal antibody (66059-1-Ig)

    IHC staining of human liver cancer using 66059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RBM15 Monoclonal antibody (66059-1-Ig)

    IHC staining of human breast cancer using 66059-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM15 Monoclonal antibody (66059-1-Ig)

    IF Staining of HeLa using 66059-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, human brain tissue, HeLa cells, human heart tissue, HEK-293 cells, HEK293 cells, MCF-7 cells, LNCaP cells, Jurkat cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66059-1-Ig targets RBM15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RBM15 fusion protein Ag17970
Full Name RNA binding motif protein 15
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC006397
Gene Symbol RBM15
Gene ID (NCBI) 64783
RRIDAB_11042326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Protocols

Product Specific Protocols
WB protocol for RBM15 antibody 66059-1-IgDownload protocol
IHC protocol for RBM15 antibody 66059-1-IgDownload protocol
IF protocol for RBM15 antibody 66059-1-IgDownload protocol
IP protocol for RBM15 antibody 66059-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Commun

Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.

Authors - Yuxian Guo
View Article
humanWB

Elife

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

Authors - Li Zhang
View Article
WB

EMBO Rep

The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.

Authors - Ngoc-Tung Tran
View Article
humanIHC

J Gene Med

The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.

Authors - Yujie Li
View Article