Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of A549 using 66015-2-Ig
WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.
WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.
WB analysis using 66015-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 66015-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens).
FC experiment of HeLa using 66015-2-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
66015-2-Ig targets RBBP9 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of A549 using 66015-2-Ig
WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.
WB analysis using 66015-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 66015-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas cancer using 66015-2-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens).
FC (INTRA) Figures
FC experiment of HeLa using 66015-2-Ig
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
RBBP9 Monoclonal antibody
Catalog Number
66015-2-Ig
Citations
-
Dilutions
WB : 1:2000-1:10000 IHC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, FC (Intra), ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
