RAP2A Monoclonal antibody, PBS Only

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  WB analysis using 68368-1-Ig (same clone as 68368-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68368-1-Ig (RAP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68368-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 68368-1-Ig (same clone as 68368-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAP2A antibody (68368-1-Ig, Clone: 1F6F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68368-1-PBS in a different storage buffer formulation.

RAP2A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68368-1-PBS

Clone No.1F6F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RbBP 30, Ras-related protein Rap-2a, Ras related protein Rap 2a, RAP2, EC:3.6.5.2

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Product Information

68368-1-PBS targets RAP2A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAP2A fusion protein Ag4979
• Full Name: RAP2A, member of RAS oncogene family
• Calculated Molecular Weight: 183 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC041333
• Gene Symbol: RAP2A
• Gene ID (NCBI): 5911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P10114
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ras-related protein Rap-2a (RAP2A) is also named as RbBP-30, and belongs to the small GTPase superfamily and Ras family. RAP2A is small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form, RAP2A can interact with and regulates several effectors including MAP4K4, MINK1 and TNIK. a Nedd4-1/Rap2A/TNIK signaling pathway regulates neurite growth and arborization in mammalian neurons (PMID:20159449). Early research indicated that RAP2A is mainly located in the Golgi apparatus of mammalian cells. RAP2A functions in several cascade signals to regulate biological behaviors such as cytoskeletal rearrangement, cell growth and apoptosis, and cell adhesion and migration (PMID:34018090, PMID:16246175, PMID: 16540189).