Various lysates were subjected to SDS PAGE followed by western blot with 68368-1-Ig (RAP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68368-1-Ig (RAP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF Staining of MCF-7 using 68368-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAP2A antibody (68368-1-Ig, Clone: 1F6F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Ras-related protein Rap-2a (RAP2A) is also named as RbBP-30, and belongs to the small GTPase superfamily and Ras family. RAP2A is small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form, RAP2A can interact with and regulates several effectors including MAP4K4, MINK1 and TNIK. a Nedd4-1/Rap2A/TNIK signaling pathway regulates neurite growth and arborization in mammalian neurons (PMID:20159449). Early research indicated that RAP2A is mainly located in the Golgi apparatus of mammalian cells. RAP2A functions in several cascade signals to regulate biological behaviors such as cytoskeletal rearrangement, cell growth and apoptosis, and cell adhesion and migration (PMID:34018090, PMID:16246175, PMID: 16540189).
Various lysates were subjected to SDS PAGE followed by western blot with 68368-1-Ig (RAP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
IF/ICC Figures
IF Staining of MCF-7 using 68368-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAP2A antibody (68368-1-Ig, Clone: 1F6F3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RAP2A Monoclonal antibody
Catalog Number
68368-1-Ig
Citations
-
Dilutions
WB : 1:2000-1:10000 IF/ICC : 1:200-1:800
Applications
WB, IF/ICC, ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
