CoraLite® Plus 488-conjugated RAP1GAP Polyclonal antibody

RAP1GAP Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19174

Synonyms

RAPGAP, RAP1GAP1, RAP1GA1, Rap1 GTPase-activating protein 1, RAP1 GTPase activating protein

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Validation Data Gallery

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  IF Staining of HeLa using CL488-19174

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RAP1GAP antibody (CL488-19174) at dilution of 1:200.

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  FC experiment of HeLa using CL488-19174

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Rap1gap Polyclonal Antibody (CL488-19174)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19174 targets RAP1GAP in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAP1GAP fusion protein Ag5576
• Full Name: RAP1 GTPase activating protein
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 85-95 kDa
• GenBank Accession Number: BC054490
• Gene Symbol: RAP1GAP
• Gene ID (NCBI): 5909
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.