RAP1A Monoclonal antibody, PBS Only

RAP1A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12D8

Cat no : 68125-1-PBS

Synonyms

C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21

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Validation Data Gallery

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  WB analysis using 68125-1-Ig (same clone as 68125-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68125-1-Ig (RAP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68125-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68125-1-Ig (same clone as 68125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68125-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68125-1-Ig (same clone as 68125-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68125-1-PBS in a different storage buffer formulation.

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Product Information

68125-1-PBS targets RAP1A in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAP1A fusion protein Ag9463
• Full Name: RAP1A, member of RAS oncogene family
• Calculated Molecular Weight: 184 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC014086
• Gene Symbol: RAP1A
• Gene ID (NCBI): 5906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).