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RAP1A Monoclonal antibody

RAP1A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12D8

Cat no : 68125-1-Ig

Synonyms

C21KG, G 22K, GTP binding protein smg p21A, KREV 1, KREV1, RAP1, RAP1A, Ras related protein Krev 1, Ras related protein Rap 1A, SMGP21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1A Monoclonal antibody (68125-1-Ig)

    WB analysis using 68125-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68125-1-Ig (RAP1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAP1A Monoclonal antibody (68125-1-Ig)

    IHC staining of human liver cancer using 68125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAP1A Monoclonal antibody (68125-1-Ig)

    IHC staining of human liver cancer using 68125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68125-1-Ig (RAP1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, Raji cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68125-1-Ig targets RAP1A in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RAP1A fusion protein Ag9463
Full Name RAP1A, member of RAS oncogene family
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

Protocols

Product Specific Protocols
WB protocol for RAP1A antibody 68125-1-IgDownload protocol
IHC protocol for RAP1A antibody 68125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autoimmunity

RGS7 silence protects palmitic acid-induced pancreatic β-cell injury by inactivating the chemokine signaling pathway

Authors - Yurong Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Guorong (Verified Customer) (11-22-2022)

Multiple bands distributed between 50-150 kDa were observed . Does not represent the performance in other kind of samples

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: ARPE-19
RAP1A Antibody Western Blot validation (1:2000 dilution) in ARPE-19 (Cat no:68125-1-Ig)