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CoraLite® Plus 488-conjugated RALBP1 Monoclonal antibody

RALBP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2F5

Cat no : CL488-67359

Synonyms

DNP-SG ATPase, DNP SG ATPase, Dinitrophenyl S-glutathione ATPase, 76 kDa Ral-interacting protein, 76 kDa Ral interacting protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated RALBP1 Monoclonal an (CL488-67359)

    IF Staining of A431 using CL488-67359

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 RALBP1 antibody (CL488-67359, Clone: 1B2F5 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated RALBP1 Monoclonal an (CL488-67359)

    FC experiment of HEK-293 using CL488-67359

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RALBP1 (CL488-67359, Clone:1B2F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67359 targets RALBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RALBP1 fusion protein Ag28618
Full Name ralA binding protein 1
Calculated Molecular Weight 655 aa, 76 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC013126
Gene Symbol RALBP1
Gene ID (NCBI) 10928
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RALBP1 antibody CL488-67359Download protocol
Standard Protocols
Click here to view our Standard Protocols