RALA Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RALA Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H9H5

Cat no : 67093-1-PBS

Synonyms

RAL, RALA, Ras related protein Ral A

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Validation Data Gallery

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  WB analysis using 67093-1-Ig (same clone as 67093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67093-1-Ig (same clone as 67093-1-PBS)

  WB result of RALA antibody (67093-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67093-1-Ig (same clone as 67093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67093-1-Ig (same clone as 67093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67093-1-Ig (same clone as 67093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67093-1-PBS targets RALA in WB, IHC, Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RALA fusion protein Ag5329
• Full Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Calculated Molecular Weight: 206 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC039858
• Gene Symbol: RALA
• Gene ID (NCBI): 5898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.