RALA Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RALA Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2a

Reactivity

pig, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

1H9H5

Cat no : 67093-1-PBS

Synonyms

Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5

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Validation Data Gallery

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  WB analysis using 67093-1-Ig (same clone as 67093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67093-1-Ig (same clone as 67093-1-PBS)

  WB result of RALA antibody (67093-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67093-1-Ig (same clone as 67093-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67093-1-Ig (same clone as 67093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67093-1-Ig (same clone as 67093-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 67093-1-Ig (same clone as 67093-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RALA (67093-1-Ig, Clone:1H9H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67093-1-PBS in a different storage buffer formulation.

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Product Information

67093-1-PBS targets RALA in Indirect ELISA, IHC, WB applications and shows reactivity with pig, human samples.

• Tested Reactivity: pig, human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RALA fusion protein Ag5329
• Full Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Calculated Molecular Weight: 206 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC039858
• Gene Symbol: RALA
• Gene ID (NCBI): 5898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.