RALA Monoclonal antibody

• Featured Product
• KD/KO Validated

RALA Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugate

Unconjugated

Publications

1

CloneNo.

1H9H5

Cat no : 67093-1-Ig

Synonyms

Ras-related protein Ral-A, Ras related protein Ral A, RAL, EC:3.6.5.2, 1H9H5

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Validation Data Gallery

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  WB analysis using 67093-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67093-1-Ig

  WB result of RALA antibody (67093-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RALA transfected HeLa cells.

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  WB analysis using 67093-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67093-1-Ig (RALA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67093-1-Ig (RALA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MCF-7 using 67093-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human RALA (67093-1-Ig, Clone:1H9H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, pig brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67093-1-Ig targets RALA in WB, IHC, FC (Intra), ELISA, IF applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RALA fusion protein Ag5329
• Full Name: v-ral simian leukemia viral oncogene homolog A (ras related)
• Calculated Molecular Weight: 206 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC039858
• Gene Symbol: RALA
• Gene ID (NCBI): 5898
• RRID: AB_2882398
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cancers (Basel); Controlled Plasma Membrane Delivery of FGFR1 and Modulation of Signaling by a Novel Regulated Anterograde RTK Transport Pathway; Authors - Claire Leist Hinsch