RAG2 Monoclonal antibody, PBS Only

RAG2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66998-1-PBS
Clone No.1B4E5

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

66998-1-PBS targets RAG2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RAG2 fusion protein Ag16780 Predict reactive species
Full Name recombination activating gene 2
Calculated Molecular Weight 527 aa, 59 kDa
Observed Molecular Weight 57-62 kDa
GenBank Accession NumberBC022397
Gene Symbol RAG2
Gene ID (NCBI) 5897
RRIDAB_2882315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55895
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3

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