RAG2 Monoclonal antibody, PBS Only

RAG2 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4E5

Cat no : 66998-1-PBS

Synonyms

V(D)J recombination-activating protein 2, RAG-2, RAG 2, 1B4E5

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Validation Data Gallery

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  WB analysis using 66998-1-Ig (same clone as 66998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66998-1-Ig (RAG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66998-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66998-1-Ig (same clone as 66998-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAG2 antibody (66998-1-Ig, Clone: 1B4E5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66998-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66998-1-Ig (same clone as 66998-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RAG2 (66998-1-Ig, Clone:1B4E5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66998-1-PBS in a different storage buffer formulation.

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Product Information

66998-1-PBS targets RAG2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAG2 fusion protein Ag16780
• Full Name: recombination activating gene 2
• Calculated Molecular Weight: 527 aa, 59 kDa
• Observed Molecular Weight: 57-62 kDa
• GenBank Accession Number: BC022397
• Gene Symbol: RAG2
• Gene ID (NCBI): 5897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3