Product Information
66998-1-PBS targets RAG2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human, pig samples.
| Tested Reactivity | Human, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RAG2 fusion protein Ag16780 Predict reactive species |
| Full Name | recombination activating gene 2 |
| Calculated Molecular Weight | 527 aa, 59 kDa |
| Observed Molecular Weight | 57-62 kDa |
| GenBank Accession Number | BC022397 |
| Gene Symbol | RAG2 |
| Gene ID (NCBI) | 5897 |
| RRID | AB_2882315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55895 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3
