RAG1 Monoclonal antibody, PBS Only

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  WB analysis using 68591-1-Ig (same clone as 68591-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 68591-1-Ig (RAG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68591-1-PBS in a different storage buffer formulation.

RAG1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68591-1-PBS

Clone No.3A1A4

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Synonyms

RAG 1, RAG1, RNF74

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Product Information

68591-1-PBS targets RAG1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAG1 fusion protein Ag30192
• Full Name: recombination activating gene 1
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 110-119 kDa
• GenBank Accession Number: NM_000448
• Gene Symbol: RAG1
• Gene ID (NCBI): 5896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15918
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Recombination-activating gene (RAG) proteins include RAG1 and RAG2, which are crucial for Ig and T lymphocyte receptor (TCR) fragment rearrangement and are responsible for DNA recognition and cleavage at specific sequences. Mutations in RAG1 result in the complete or partial loss of recombinant enzyme activity, in unbalanced variable-diversity-joining (V(D)J) recombination, and in the impairment of T and B lymphocyte development at the early stages.