RAG1 Monoclonal antibody

• 

  WB analysis using 68591-1-Ig

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 68591-1-Ig (RAG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RAG1 Monoclonal Antibody for WB, ELISA

Cat No. 68591-1-Ig

Clone No.3A1A4

Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

RAG 1, RAG1, RNF74

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Tested Applications

• Positive WB detected in: mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68591-1-Ig targets RAG1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAG1 fusion protein Ag30192
• Full Name: recombination activating gene 1
• Calculated Molecular Weight: 119 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: NM_000448
• Gene Symbol: RAG1
• Gene ID (NCBI): 5896
• RRID: AB_3085290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15918
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Recombination-activating gene (RAG) proteins include RAG1 and RAG2, which are crucial for Ig and T lymphocyte receptor (TCR) fragment rearrangement and are responsible for DNA recognition and cleavage at specific sequences. Mutations in RAG1 result in the complete or partial loss of recombinant enzyme activity, in unbalanced variable-diversity-joining (V(D)J) recombination, and in the impairment of T and B lymphocyte development at the early stages.

Publications

Species	Application	Title
		Int J Biol Macromol; The pivotal role of CRIHSP sequences in orchestrating antigen receptor diversity and genomic stability within antigen receptor germline genes; Authors - Zhiyuan Niu