CoraLite®594-conjugated RAF1 Monoclonal antibody

• Featured Product
• KD/KO Validated

RAF1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D6A1

Cat no : CL594-66592

Synonyms

c Raf, CRAF, NS5, Proto oncogene c RAF, RAF, Raf 1, RAF1

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Validation Data Gallery

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  IF Staining of HEK-293 using CL594-66592

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®®594 RAF1 antibody (CL594-66592, Clone: 1D6A1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66592 targets RAF1 in IF/ICC applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAF1 fusion protein Ag25423
• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• RRID: AB_2920013
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.