RAF1 Recombinant antibody

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  WB analysis using 84994-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84994-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84994-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

RAF1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84994-4-RR

Clone No.242540A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

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Tested Applications

• Positive WB detected in: HCT 116 cells, K-562 cells, HeLa cells, DU 145 cells, NIH/3T3 cells, PC-12 cells, RAW 264.7 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84994-4-RR targets RAF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAF1 fusion protein Ag25423
• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: ~75 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04049
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.