RAF1 Recombinant antibody, PBS Only

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  WB analysis using 84994-4-RR (same clone as 84994-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84994-4-RR (RAF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84994-4-RR (same clone as 84994-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAF1 antibody (84994-4-RR, Clone: 242540A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84994-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84994-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84994-4-RR against Human RAF1 were performed. The affinity constant is 2.84 nM.

RAF1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84994-4-PBS

Clone No.242540A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF, c Raf

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Product Information

84994-4-PBS targets RAF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAF1 fusion protein Ag25423
• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: ~75 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04049
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.