RAE1 Recombinant antibody

RAE1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240861B7

Cat no : 83712-4-RR

Synonyms

mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14

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Validation Data Gallery

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  WB analysis using 83712-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83712-4-RR (RAE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83712-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAE1 antibody (83712-4-RR, Clone: 240861B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83712-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83712-4-RR against Human RAE1 were performed. The affinity constant is 20.7 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, BxPC-3 cells, mouse thymus tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83712-4-RR is RAE1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAE1 fusion protein Ag12102
• Full Name: RAE1 RNA export 1 homolog (S. pombe)
• Calculated Molecular Weight: 368 aa, 41 kDa
• GenBank Accession Number: BC103754
• Gene Symbol: RAE1
• Gene ID (NCBI): 8480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAE1 acts as a mRNA export factor involved in nucleocytoplasmic transport, it plays a role in mitotic bipolar spindle formation. (PMID: 33849972, MID: 17172455)