RAD9A Polyclonal antibody, PBS Only

RAD9A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13035-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 13035-1-AP (same clone as 13035-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 13035-1-AP (same clone as 13035-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13035-1-AP (same clone as 13035-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13035-1-AP (same clone as 13035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13035-1-AP (same clone as 13035-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13035-1-AP (same clone as 13035-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13035-1-PBS in a different storage buffer formulation.

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Product Information

13035-1-PBS targets RAD9A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD9A fusion protein Ag3687
• Full Name: RAD9 homolog A (S. pombe)
• Calculated Molecular Weight: 391 aa, 42 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC014848
• Gene Symbol: RAD9A
• Gene ID (NCBI): 5883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.