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RAD9A Polyclonal antibody

RAD9A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13035-1-AP

Synonyms

RAD9, hRAD9, EC:3.1.11.2, Cell cycle checkpoint control protein RAD9A

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RAD9A Polyclonal antibody (13035-1-AP)

    WB analysis of HeLa using 13035-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RAD9A Polyclonal antibody (13035-1-AP)

    WB analysis of A549 using 13035-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RAD9A Polyclonal antibody (13035-1-AP)

    WB analysis of MCF-7 using 13035-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13035-1-AP (RAD9A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD9A Polyclonal antibody (13035-1-AP)

    IHC staining of human breast cancer using 13035-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD9A Polyclonal antibody (13035-1-AP)

    IHC staining of human breast cancer using 13035-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13035-1-AP (RAD9A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD9A Polyclonal antibody (13035-1-AP)

    IF Staining of HeLa using 13035-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13035-1-AP (RAD9A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

13035-1-AP targets RAD9A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD9A fusion protein Ag3687
Full Name RAD9 homolog A (S. pombe)
Calculated Molecular Weight 391 aa, 42 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC014848
Gene Symbol RAD9A
Gene ID (NCBI) 5883
RRIDAB_10598474
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAD9A antibody 13035-1-APDownload protocol
IHC protocol for RAD9A antibody 13035-1-APDownload protocol
IF protocol for RAD9A antibody 13035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

YTHDC1 delays cellular senescence and pulmonary fibrosis by activating ATR in an m6A-independent manner

Authors - Canfeng Zhang
View Article
humanWB

Oncogene

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen
View Article
humanWB

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu
View Article
humanWB

DNA Cell Biol

AEG-1 Knockdown Sensitizes Glioma Cells to Radiation Through Impairing Homologous Recombination Via Targeting RFC5.

Authors - Xu Zhao
View Article