CoraLite® Plus 488-conjugated RAD21 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAD21 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27071

Synonyms

FLJ25655, FLJ40596, hHR21, HR21, HRAD21, KIAA0078, MCD1, Nuclear matrix protein 1, NXP 1, NXP1, RAD21, RAD21 homolog (S. pombe), SCC1, SCC1 homolog

---

Validation Data Gallery

• 

  IF Staining of MDCK using CL488-27071

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CoraLite® Plus 488 RAD21 antibody (CL488-27071) at dilution of 1:200.

---

Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-27071 targets RAD21 in IF/ICC applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAD21 fusion protein Ag25481
• Full Name: RAD21 homolog (S. pombe)
• Calculated Molecular Weight: 631 aa, 72 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC050381
• Gene Symbol: RAD21
• Gene ID (NCBI): 5885
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells