RACK1; GNB2L1 Monoclonal antibody, PBS Only

RACK1; GNB2L1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11A7

Cat no : 66940-1-PBS

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

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Validation Data Gallery

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  WB analysis using 66940-1-Ig (same clone as 66940-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66940-1-Ig (same clone as 66940-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66940-1-Ig (same clone as 66940-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66940-1-Ig (same clone as 66940-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66940-1-Ig (same clone as 66940-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66940-1-Ig (same clone as 66940-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66940-1-Ig (same clone as 66940-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RACK1; GNB2L1 antibody (66940-1-Ig, Clone: 1E11A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66940-1-PBS in a different storage buffer formulation.

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Product Information

66940-1-PBS targets RACK1; GNB2L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC019093
• Gene Symbol: RACK1
• Gene ID (NCBI): 10399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).