RACK1; GNB2L1 Monoclonal antibody

RACK1; GNB2L1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1E11A7

Cat no : 66940-1-Ig

Synonyms

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1

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Validation Data Gallery

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  WB analysis using 66940-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66940-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66940-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66940-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66940-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66940-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66940-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66940-1-Ig (RACK1; GNB2L1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66940-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RACK1; GNB2L1 antibody (66940-1-Ig, Clone: 1E11A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, pig liver tissue, NIH/3T3 cells, HSC-T6 cells, HepG2 cells, T-47D cells, L02 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

66940-1-Ig targets RACK1; GNB2L1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RACK1; GNB2L1 fusion protein Ag26708
• Full Name: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC019093
• Gene Symbol: RACK1
• Gene ID (NCBI): 10399
• RRID: AB_2882264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publications

Species	Application	Title
human	IF	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang
human	WB,IHC,IF,IP,CoIP	Cell Death Differ; Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression; Authors - Yanan Pi
mouse,rat	IP,WB	Am J Physiol Renal Physiol; Neuropilin 1 Promotes Unilateral Ureteral Obstruction-Induced Renal Fibrosis via RACK1 in Renal Tubular Epithelial Cells; Authors - Ling Hou
human	WB,CoIP	ACS Med Chem Lett; Photoaffinity Probe Reveals the Potential Target of Harringtonolide for Cancer Cell Migration Inhibition; Authors - Tian-Yu Zhu