CoraLite® Plus 488-conjugated RAB5A Monoclonal antibody

RAB5A Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6A5

Cat no : CL488-66339

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

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Validation Data Gallery

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  IF Staining of HeLa using CL488-66339

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAB5A antibody (CL488-66339, Clone: 1B6A5 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66339 targets RAB5A in IF/ICC applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB5A fusion protein Ag16781
• Full Name: RAB5A, member RAS oncogene family
• Calculated Molecular Weight: 215 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001267
• Gene Symbol: RAB5A
• Gene ID (NCBI): 5868
• RRID: AB_2883313
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion. This anitbody is CL488 conjugated.