RAB5A Monoclonal antibody

RAB5A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1B6A5

Cat no : 66339-1-Ig

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

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Validation Data Gallery

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  WB analysis of HEK-293 using 66339-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66339-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 66339-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 66339-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66339-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66339-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66339-1-Ig(RAB5A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, fetal human brain tissue, pig brain tissue
• Positive IHC detected in: human liver cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

66339-1-Ig targets RAB5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, monkey
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB5A fusion protein Ag16781
• Full Name: RAB5A, member RAS oncogene family
• Calculated Molecular Weight: 215 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC001267
• Gene Symbol: RAB5A
• Gene ID (NCBI): 5868
• RRID: AB_2881719
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.

Publications

Species	Application	Title
human	IF	Bioact Mater; Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis.; Authors - Jin Wang
human	IF	Clin Ther; Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019.; Authors - Yoel Benarroch
human	WB	J Exp Clin Cancer Res; TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells.; Authors - Ruizhi He
		Commun Biol; Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.; Authors - Kun Meng
monkey	WB	PLoS Pathog; ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses; Authors - Xiongnan Chen
human	IHC	Cell Rep; Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina; Authors - Yacine Touahri