CoraLite® Plus 488-conjugated RAB3GAP1 Monoclonal antibody

RAB3GAP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A3A1

Cat no : CL488-66688

Synonyms

RAB3 GTPase-activating protein 130 kDa subunit, Rab3 GAP p130, Rab3 GAP, P130, KIAA0066

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-66688

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 RAB3GAP1 antibody (CL488-66688, Clone: 4A3A1 ) at dilution of 1:100.

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  FC experiment of HEK-293 using CL488-66688

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RAB3GAP1 (CL488-66688, Clone:4A3A1) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66688 targets RAB3GAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB3GAP1 fusion protein Ag17710
• Full Name: RAB3 GTPase activating protein subunit 1 (catalytic)
• Calculated Molecular Weight: 981 aa, 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC146809
• Gene Symbol: RAB3GAP1
• Gene ID (NCBI): 22930
• RRID: AB_2919358
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.