RAB32 Recombinant antibody, PBS Only

RAB32 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230315B10

Cat no : 82960-5-PBS

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

---

Validation Data Gallery

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 82960-5-RR (same clone as 82960-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 82960-5-RR (same clone as 82960-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82960-5-RR (same clone as 82960-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 82960-5-RR (same clone as 82960-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB32 Recombinant antibody (82960-5-RR, Clone:230315B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82960-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82960-5-RR against Human RAB32 were performed. The affinity constant is 0.499 nM.

---

Product Information

The immunogen of 82960-5-PBS is RAB32 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RAB32 fusion protein Ag1450
• Full Name: RAB32, member RAS oncogene family
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: BC015061
• Gene Symbol: RAB32
• Gene ID (NCBI): 10981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.