Filter: AllIHCIF/ICCFC (Intra)Affinity

IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U-251 using 82960-5-RR
Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 82960-5-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
IF Staining of U-251 using 82960-5-RR
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 82960-5-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB32 Recombinant antibody (82960-5-RR, Clone:230315B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 82960-5-RR
Biolayer interferometry (BLl) kinetic assays of 82960-5-RR against Human RAB32 were performed. The affinity constant is 0.499 nM.

RAB32 Recombinant antibody

RAB32 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 82960-5-RR

Clone No.230315B10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U-251 cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82960-5-RR targets RAB32 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	RAB32 fusion protein Ag1450 Predict reactive species
Full Name	RAB32, member RAS oncogene family
Calculated Molecular Weight	25 kDa
GenBank Accession Number	BC015061
Gene Symbol	RAB32
Gene ID (NCBI)	10981
RRID	AB_3670706
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q13637
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

Protocols

Product Specific Protocols
IHC protocol for RAB32 antibody 82960-5-RR	Download protocol
IF protocol for RAB32 antibody 82960-5-RR	Download protocol
FC protocol for RAB32 antibody 82960-5-RR	Download protocol