RAB2A Monoclonal antibody, PBS Only

RAB2A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E1B10

Cat no : 67501-1-PBS

Synonyms

RAB2, RAB2A, Ras related protein Rab 2A

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Validation Data Gallery

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  WB analysis using 67501-1-Ig (same clone as 67501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67501-1-Ig (RAB2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67501-1-Ig (same clone as 67501-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67501-1-Ig (same clone as 67501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67501-1-Ig (same clone as 67501-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67501-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67501-1-PBS targets RAB2A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB2A fusion protein Ag7939
• Full Name: RAB2A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC008929
• Gene Symbol: RAB2A
• Gene ID (NCBI): 5862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B.