RAB2A Monoclonal antibody

RAB2A Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3E1B10

Cat no : 67501-1-Ig

Synonyms

RAB2, Ras-related protein Rab-2A, Ras related protein Rab 2A, EC:3.6.5.2, 3E1B10

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Validation Data Gallery

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  WB analysis using 67501-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67501-1-Ig (RAB2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67501-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67501-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67501-1-Ig (RAB2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67501-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse brain using 67501-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of U2OS using 67501-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67501-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RAB2A antibody (67501-1-Ig, Clone: 3E1B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: U2OS cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: U2OS cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67501-1-Ig targets RAB2A in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB2A fusion protein Ag7939
• Full Name: RAB2A, member RAS oncogene family
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC008929
• Gene Symbol: RAB2A
• Gene ID (NCBI): 5862
• RRID: AB_2882725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein belongs to the Rab family, members of which are small molecular weight guanosine triphosphatases (GTPases) that contain highly conserved domains involved in GTP binding and hydrolysis. The Rabs are membrane-bound proteins, involved in vesicular fusion and trafficking. This protein is a resident of pre-Golgi intermediates, and is required for protein transport from the endoplasmic reticulum (ER) to the Golgi complex. This antibody can recognize RAB2A and RAB2B.

Publications

Species	Application	Title
mouse	IF	Front Physiol; Upregulation of Amy1 in the salivary glands of mice exposed to a lunar gravity environment using the multiple artificial gravity research system; Authors - Takehito Ouchi