RAB27B Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAB27B Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B9G9

Cat no : 66944-1-PBS

Synonyms

C25KG, RAB27B, Ras related protein Rab 27B

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Validation Data Gallery

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  WB analysis using 66944-1-Ig (same clone as 66944-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 66944-1-Ig (same clone as 66944-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66944-1-Ig (same clone as 66944-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66944-1-Ig (same clone as 66944-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 66944-1-Ig (same clone as 66944-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB27B (66944-1-Ig, Clone:2B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66944-1-PBS targets RAB27B in WB, FC, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB27B fusion protein Ag18756
• Full Name: RAB27B, member RAS oncogene family
• Calculated Molecular Weight: 218 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC027474
• Gene Symbol: RAB27B
• Gene ID (NCBI): 5874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.