RAB27A Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RAB27A Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4B8

Cat no : 66058-1-PBS

Synonyms

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

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Validation Data Gallery

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  WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

  WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66058-1-Ig (same clone as 66058-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66058-1-PBS targets RAB27A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB27A fusion protein Ag12382
• Full Name: RAB27A, member RAS oncogene family
• Calculated Molecular Weight: 221 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC107680
• Gene Symbol: RAB27A
• Gene ID (NCBI): 5873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.