RAB11A Monoclonal antibody

RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

4A4C9

Reviews (1)

Cat no : 67902-1-Ig

Synonyms

RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2

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Validation Data Gallery

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  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of mouse brain using 67902-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67902-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67902-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 67902-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HeLa using 67902-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RAB11A Monoclonal antibody (67902-1-Ig, Clone:4A4C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A431 cells, A549 cells, HeLa cells, pig brain tissue, rat brain tissue, rat cerebellum tissue, HepG2 cells, K-562 cells, rabbit brain tissue
• Positive IF/ICC detected in: A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

67902-1-Ig targets RAB11A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RAB11A fusion protein Ag8879
• Full Name: RAB11A, member RAS oncogene family
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC013348
• Gene Symbol: RAB11A
• Gene ID (NCBI): 8766
• RRID: AB_2918658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

Publications

Species	Application	Title
human	IF	Cell Rep; A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma; Authors - Hongli Zhang
	WB,IF	Cell Commun Signal; Atg5-deficient mesenchymal stem cells protect against non-alcoholic fatty liver by accelerating hepatocyte growth factor secretion; Authors - Caifeng Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (10-17-2024)

A very good antibody to detect endo Rab11A from several sources.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

RAB11A Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:67902-1-Ig)