RAB10 Polyclonal antibody

• Featured Product
• KD/KO Validated

RAB10 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27094-1-AP

Synonyms

RAB10, Ras related protein Rab 10

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Validation Data Gallery

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  WB analysis of MDCK using 27094-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27094-1-AP

  WB result of RAB10 antibody (27094-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.

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  WB analysis of MCF-7 using 27094-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27094-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27094-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27094-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 27094-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27094-1-AP (RAB10 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of NIH/3T3 using 27094-1-AP

  Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 27094-1-AP (RAB10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 27094-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RAB10 (27094-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, MDCK cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

27094-1-AP targets RAB10 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAB10 fusion protein Ag25830
• Full Name: RAB10, member RAS oncogene family
• Calculated Molecular Weight: 200 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC000896
• Gene Symbol: RAB10
• Gene ID (NCBI): 10890
• RRID: AB_2880752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
mouse	IF	Biochem Biophys Res Commun; Extracellular HSPA5 is autocrinally involved in the regulation of neuronal process elongation; Authors - Miku Fukawa