Protein A Monoclonal antibody, PBS Only

• 

  WB analysis of ProteinA using 66945-1-Ig (same clone as 66945-1-PBS)

  Commercial Protein A and negative samples (human plasma, HeLa cells, rat liver, mouse brain) were subjected to SDS-PAGE followed by western blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66945-1-PBS in a different storage buffer formulation.

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  WB analysis of Recombinant protein using 66945-1-Ig (same clone as 66945-1-PBS)

  Commercial Protein A was deactivated and lysised followed by SDS-PAGE and then blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 and isotype control antibody 66360-1-Ig at 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66945-1-PBS in a different storage buffer formulation.

Protein A Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66945-1-PBS

Clone No.1G6A6

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

staphylococcus aureus and More (1)

Applications

WB, Indirect ELISA

Synonyms

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Published Applications

• WB: See 1 publications below

Product Information

66945-1-PBS targets Protein A in WB, Indirect ELISA applications and shows reactivity with staphylococcus aureus samples.

• Tested Reactivity: staphylococcus aureus
• Cited Reactivity: yeast
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Protein A
• GenBank Accession Number: EU695225
• RRID: AB_2882269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Publications

Species	Application	Title
yeast	WB	Proc Natl Acad Sci U S A; Mec1-mediated Atg9 phosphorylation regulates the PAS recruitment of Atg9 vesicles upon energy stress; Authors - Siyu Fan