CALCA/CGRP Recombinant antibody, PBS Only (Capture)

CALCA/CGRP Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240753G2

Cat no : 83774-1-PBS

Synonyms

Procalcitonin/CALCA, Calcitonin, CALCA, CALC1, Alpha-type CGRP

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Validation Data Gallery

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  IF Staining of TT using 83774-1-RR (same clone as 83774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed TT cells using Procalcitonin/CALCA antibody (83774-1-RR, Clone: 240753G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).(negative control: HeLa cells) This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

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  FC experiment of TT using 83774-1-RR (same clone as 83774-1-PBS)

  1x10^6 TT cells were intracellularly stained with 0.25 ug Procalcitonin/CALCA Recombinant Antibody (83774-1-RR, Clone:240753G2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00728-1

  Cytometric bead array standard curve of MP00728-1, Procalcitonin/CALCA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83774-1-PBS. Detection antibody: 83774-2-PBS. Standard: Eg1060. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83774-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83774-1-RR against Human CALCA/CGRP were performed. The affinity constant is 0.189 nM.

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Product Information

83774-1-PBS targets CALCA/CGRP as part of a matched antibody pair:

MP00728-1: 83774-1-PBS capture and 83774-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: calcitonin-related polypeptide alpha
• Calculated Molecular Weight: 15kDa
• GenBank Accession Number: NM_001741.3
• Gene Symbol: CGRP
• Gene ID (NCBI): 796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).