Plasminogen Polyclonal antibody, PBS Only

Plasminogen Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18300-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18300-1-AP (same clone as 18300-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18300-1-AP (same clone as 18300-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18300-1-AP (same clone as 18300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18300-1-AP (same clone as 18300-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 18300-1-AP (same clone as 18300-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18300-1-AP (Plasminogen antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18300-1-PBS in a different storage buffer formulation.

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Product Information

18300-1-PBS targets Plasminogen in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: plasminogen
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC060513
• Gene Symbol: Plasminogen
• Gene ID (NCBI): 5340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.