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Plasminogen Polyclonal antibody

Plasminogen Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18300-1-AP

Synonyms

PLG, Plasmin heavy chain A, short form, EC:3.4.21.7, Angiostatin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Plasminogen Polyclonal antibody (18300-1-AP)

    WB analysis using 18300-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Plasminogen Polyclonal antibody (18300-1-AP)

    WB analysis of HepG2 using 18300-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18300-1-AP (Plasminogen antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Plasminogen Polyclonal antibody (18300-1-AP)

    IHC staining of human liver using 18300-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Plasminogen Polyclonal antibody (18300-1-AP)

    IHC staining of human liver using 18300-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18300-1-AP (Plasminogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Plasminogen Polyclonal antibody (18300-1-AP)

    IF Staining of HepG2 using 18300-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18300-1-AP (Plasminogen antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

18300-1-AP targets Plasminogen in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name plasminogen
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC060513
Gene Symbol Plasminogen
Gene ID (NCBI) 5340
RRIDAB_2167096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.

Protocols

Product Specific Protocols
WB protocol for Plasminogen antibody 18300-1-APDownload protocol
IHC protocol for Plasminogen antibody 18300-1-APDownload protocol
IF protocol for Plasminogen antibody 18300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Int Immunol

Zoobiquity experiments show the importance of the local MMP9-plasminogen axis in inflammatory bowel diseases in both dogs and patients

Authors - Takeshi Yamasaki
View Article