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CoraLite®594-conjugated Phospho-mTOR (Ser2448) Monoclonal antibody

Phospho-mTOR (Ser2448) Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A12G3

Cat no : CL594-67778

Synonyms

FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated Phospho-mTOR (Ser2448) Mon (CL594-67778)

    IF Staining of HepG2 using CL594-67778

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Phospho-mTOR (Ser2448) antibody (CL594-67778, Clone: 2A12G3 ) at dilution of 1:100. DAPI (blue)

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Phospho-mTOR (Ser2448) Mon (CL594-67778)

    FC experiment of HeLa using CL594-67778

    1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.13 ug CoraLite®594 Anti-Human Phospho-mTOR (Ser2448) (CL594-67778, Clone:2A12G3) (red), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67778 targets Phospho-mTOR (Ser2448) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated Molecular Weight 289 kDa
GenBank Accession NumberBC117166
Gene Symbol mTOR
Gene ID (NCBI) 2475
RRIDAB_2920182
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

Protocols

Product Specific Protocols
IF protocol for CL594 Phospho-mTOR (Ser2448) antibody CL594-67778Download protocol
Standard Protocols
Click here to view our Standard Protocols