CoraLite® Plus 488-conjugated Phospho-mTOR (Ser2448) Recombinant antibody

Phospho-mTOR (Ser2448) Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3L18

Cat no : CL488-80596

Synonyms

FRAP1, MTOR, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein

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Validation Data Gallery

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  IF Staining of HEK-293 using CL488-80596

  Immunofluorescent analysis of (-20°C Ethanol) fixed PMA treated HEK-293 cells using CoraLite® Plus 488 Phospho-mTOR (Ser2448) antibody (CL488-80596, Clone: 3L18 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-80596

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-mTOR (Ser2448) (CL488-80596, Clone:3L18) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

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Tested Applications

• Positive IF/ICC detected in: PMA treated HEK-293 cells
• Positive FC (Intra) detected in: Calyculin A treated HeLa cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-80596 targets Phospho-mTOR (Ser2448) in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: FK506 binding protein 12-rapamycin associated protein 1
• Calculated Molecular Weight: 289 kDa
• Observed Molecular Weight: 250-289 kDa
• GenBank Accession Number: BC117166
• Gene Symbol: mTOR
• Gene ID (NCBI): 2475
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.