Phospho-c-SRC (Tyr530) Recombinant monoclonal antibody

• 

  WB analysis using 80706-2-RR

  Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80706-2-RR (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-c-SRC (Tyr530) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 80706-2-RR

Clone No.241477A4

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

c-SRC (Tyr530), p c-SRC (Tyr530), p-c-SRC (Tyr530), Phospho c-SRC (Tyr530), Phospho-SRC (Tyr530)

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Tested Applications

• Positive WB detected in: LPS treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

80706-2-RR targets Phospho-c-SRC (Tyr530) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC011566
• Gene Symbol: SRC
• Gene ID (NCBI): 6714
• ENSEMBL Gene ID: ENSG00000197122
• RRID: AB_3670485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12931
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

c-Src is a 60 kDa protein encoded by the Src gene belonging to a family of non-RTK known as SFK. As a typical protein, the polypeptide sequence of c-Src comprises an N-terminal head and a C-terminal tail but has four Src homology (SH) domains numbered 1, 2, 3, and 4. The C-terminal tail consists of a carboxy group that is short and connected to the SH1 domain. Additionally, the C-terminal domain serves as an anchor for a phosphotyrosine moiety, specifically Tyr530 in human Src (or Tyr527 in chicken). (PMID: 38201459)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	J Ethnopharmacol; Paeoniflorin inhibits chronic restraint stress-induced progression of hepatocellular carcinoma through suppressing norepinephrine-induced activation of hepatic stellate cells via SRC/AKT/ERK pathways; Authors - Yujun Luo
mouse	WB	Neurol Res; Exploring neuroprotective effects of PP2 in ischemic stroke via bioinformatics and experimental validation; Authors - Shiyan Zhao
human	WB	Drug Resist Updat; Exosome-derived lnc-FAM72D-3 promotes lenvatinib resistance by remodeling hepatocellular carcinoma cytoskeleton via MBNL1/FAK axis; Authors - Mingbo Cao