Phospho-c-SRC (Tyr530) Recombinant antibody, PBS Only

Phospho-c-SRC (Tyr530) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241477A4

Cat no : 80706-2-PBS

Synonyms

c-SRC, SRC, p60-Src, EC:2.7.10.2, cSRC

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Validation Data Gallery

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  WB analysis using 80706-2-RR (same clone as 80706-2-PBS)

  Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80706-2-RR (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80706-2-PBS in a different storage buffer formulation.

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Product Information

80706-2-PBS targets Phospho-c-SRC (Tyr530) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC011566
• Gene Symbol: SRC
• Gene ID (NCBI): 6714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

c-Src is a 60 kDa protein encoded by the Src gene belonging to a family of non-RTK known as SFK. As a typical protein, the polypeptide sequence of c-Src comprises an N-terminal head and a C-terminal tail but has four Src homology (SH) domains numbered 1, 2, 3, and 4. The C-terminal tail consists of a carboxy group that is short and connected to the SH1 domain. Additionally, the C-terminal domain serves as an anchor for a phosphotyrosine moiety, specifically Tyr530 in human Src (or Tyr527 in chicken). (PMID: 38201459)