Phospho-ULK1 (Ser556) Recombinant antibody

Phospho-ULK1 (Ser556) Uni-rAb^TM Recombinant Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (3)

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

15

CloneNo.

2F5

Cat no : 80218-1-RR

Synonyms

ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

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Validation Data Gallery

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  WB analysis using 80218-1-RR

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control.

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  WB analysis using 80218-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control.

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  FC experiment of PC-3 using 80218-1-RR

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-ULK1 (Ser556) (80218-1-RR, Clone:2F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells
• Positive FC (Intra) detected in: Calyculin A treated PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 1 publications below

Product Information

80218-1-RR targets Phospho-ULK1 (Ser556) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: unc-51-like kinase 1 (C. elegans)
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_003565
• Gene Symbol: ULK1
• Gene ID (NCBI): 8408
• RRID: AB_2918877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)

Publications

Species	Application	Title
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
mouse	WB	JCI Insight; ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing mRNA; Authors - Honglin Liu
human	WB	Bioengineered; Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells.; Authors - Junli Zhang
human	WB	Oxid Med Cell Longev; tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTOR; Authors - Zengfa Deng
mouse	WB	Cell Death Dis; IKCa channels control breast cancer metabolism including AMPK-driven autophagy; Authors - Dominic Gross
rat	WB,IHC	Ecotoxicol Environ Saf; Role of SESTRIN2/AMPK/ULK1 pathway activation and lysosomes dysfunction in NaAsO2-induced liver injury under oxidative stress; Authors - Dingnian Bi