Phospho-TAK1 (Thr187) Recombinant antibody, PBS Only

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  WB analysis of HEK-293 using 81785-1-RR (same clone as 81785-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81785-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81785-1-RR (same clone as 81785-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 81785-1-RR (Phospho-TAK1 (Thr187) antibody) at dilution of 1:100000 incubated at room temperature for 1 hour. The membrane was stripped and re-blotted with Alpha Tubulin antibody as loading control. This data was developed using the same antibody clone with 81785-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 81785-1-RR (same clone as 81785-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.13 ug Phospho-TAK1 (Thr187) Recombinant antibody (81785-1-RR, Clone:2O6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81785-1-PBS in a different storage buffer formulation.

Phospho-TAK1 (Thr187) Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 81785-1-PBS

Clone No.2O6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

p TAK1, p TAK1 (Thr187), p TAK1 Thr187, Phospho TAK1, Phospho TAK1 (Thr187)

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Product Information

81785-1-PBS targets Phospho-TAK1 (Thr187) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase kinase 7
• Calculated Molecular Weight: 579 aa, 64 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC017715
• Gene Symbol: TAK1
• Gene ID (NCBI): 6885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43318
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.